Huaying Zhao, Ph.D.

After receiving her Ph.D. in Chemistry, with a specialization in protein biochemistry, Dr. Zhao spent two years on her post-doctoral research at the University of Maryland, where she worked on the study of protein-DNA and protein-protein interactions. Dr. Zhao joined the NIBIB as a Research Fellow in 2008. She has been working in the intramural research’s Laboratory of Cellular Imaging and Macromolecular Biophysics, Dynamics of Macromolecular Assembly Section on development of biophysical methodology for characterizing a variety of macromolecules, including proteins, nucleic acids, polymers and nanoparticles. Her current work has focused on applications of advanced techniques of biophysics and physical biochemistry, including sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation, isothermal titration calorimetry, fluorescence spectroscopy and surface plasmon resonance biosensing. Dr Zhao’s interests are in adapting these methods and further developing new biophysical approaches as necessary for the study of a variety of systems of interacting macromolecules.

Publications

Improving the thermal, radial, and temporal accuracy of the analytical ultracentrifuge through external references.
Ghirlando RBalbo APiszczek GBrown PHLewis MSBrautigam CASchuck PZhao H
Anal Biochem
2013 Sep 01

Biophysical methods for the study of protein interactions.
Schuck PZhao H
Methods
2013 Mar

Recorded scan times can limit the accuracy of sedimentation coefficients in analytical ultracentrifugation.
Zhao HGhirlando RPiszczek GCurth UBrautigam CASchuck P
Anal Biochem
2013 Jun 01

Overview of current methods in sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation.
Zhao HBrautigam CAGhirlando RSchuck P
Curr Protoc Protein Sci
2013 Feb

A comparison of binding surfaces for SPR biosensing using an antibody-antigen system and affinity distribution analysis.
Zhao HGorshkova IIFu GLSchuck P
Methods
2013 Mar

Structural basis of mouse cytomegalovirus m152/gp40 interaction with RAE1γ reveals a paradigm for MHC/MHC interaction in immune evasion.
Wang RNatarajan KRevilleza MJBoyd LFZhi LZhao HRobinson HMargulies DH
Proc Natl Acad Sci U S A
2012 Dec 18

Global multi-method analysis of affinities and cooperativity in complex systems of macromolecular interactions.
Zhao HSchuck P
Anal Chem
2012 Nov 06

Strategies for assessing proton linkage to bimolecular interactions by global analysis of isothermal titration calorimetry data.
Coussens NPSchuck PZhao H
J Chem Thermodyn
2012 Sep 01

High-precision isothermal titration calorimetry with automated peak-shape analysis.
Keller SVargas CZhao HPiszczek GBrautigam CASchuck P
Anal Chem
2012 Jun 05

Analysis of high-affinity assembly for AMPA receptor amino-terminal domains.
Zhao HBerger AJBrown PHKumar JBalbo AMay CACasillas ELaue TMPatterson GHMayer MLSchuck P
J Gen Physiol
2012 May

Structure of myostatin·follistatin-like 3: N-terminal domains of follistatin-type molecules exhibit alternate modes of binding.
Cash JNAngerman EBKattamuri CNolan KZhao HSidis YKeutmann HTThompson TB
J Biol Chem
2012 Jan 06

Density contrast sedimentation velocity for the determination of protein partial-specific volumes.
Brown PHBalbo AZhao HEbel CSchuck P
PLoS One
2011

The molecular refractive function of lens γ-Crystallins.
Zhao HBrown PHMagone MTSchuck P
J Mol Biol
2011 Aug 19

The role of macromolecular crowding in the evolution of lens crystallins with high molecular refractive index.
Zhao HMagone MTSchuck P
Phys Biol
2011 Aug

On the distribution of protein refractive index increments.
Zhao HBrown PHSchuck P
Biophys J
2011 May 04

Editorial for the special issue of methods "Modern Analytical Ultracentrifugation".
Schuck PZhao H
Methods
2011 May

The boundary structure in the analysis of reversibly interacting systems by sedimentation velocity.
Zhao HBalbo ABrown PHSchuck P
Methods
2011 May

Pre- and postexposure protection against virulent anthrax infection in mice by humanized monoclonal antibodies to Bacillus anthracis capsule.
Chen ZSchneerson RLovchik JLyons CRZhao HDai ZKubler-Kielb JLeppla SHPurcell RH
Proc Natl Acad Sci U S A
2011 Jan 11

Accounting for solvent signal offsets in the analysis of interferometric sedimentation velocity data.
Zhao HBrown PHBalbo AFernández-Alonso Mdel CPolishchuck NChaudhry CMayer MLGhirlando RSchuck P
Macromol Biosci
2010 Jul 07

The role of mass transport limitation and surface heterogeneity in the biophysical characterization of macromolecular binding processes by SPR biosensing.
Schuck PZhao H
Methods Mol Biol
2010

Assembly, loading, and alignment of an analytical ultracentrifuge sample cell.
Balbo AZhao HBrown PHSchuck P
J Vis Exp
2009 Nov 05

Potent neutralization of anthrax edema toxin by a humanized monoclonal antibody that competes with calmodulin for edema factor binding.
Chen ZMoayeri MZhao HCrown DLeppla SHPurcell RH
Proc Natl Acad Sci U S A
2009 Aug 11

Thermodynamic and structural investigation of bispecificity in protein-protein interactions.
Zhao HNaganathan SBeckett D
J Mol Biol
2009 Jun 05

Kinetic partitioning between alternative protein-protein interactions controls a transcriptional switch.
Zhao HBeckett D
J Mol Biol
2008 Jun 27

Protein-protein interactions dominate the assembly thermodynamics of a transcription repression complex.
Zhao HStreaker EPan WBeckett D
Biochemistry
2007 Nov 27

Surface-plasmon-polariton assisted diffraction in periodic subwavelength holes of metal films with reduced interplane coupling.
Fang XLi ZLong YWei HLiu RMa JKamran MZhao HHan XZhao BQiu X
Phys Rev Lett
2007 Aug 10

Effect of linker segments on the stability of epithelial cadherin Domain 2.
Prasad AZhao HRutherford JMHousley NNichols CPedigo S
Proteins
2006 Jan 01