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Dr. Schuck obtained his Ph.D. from the Goethe-University Frankfurt am Main, Germany, where he worked on interactions of integral proteins of the erythrocyte membrane using analytical ultracentrifugation. He received his post-doctoral research training in physical biochemistry with Dr. Allen Minton at NIDDK, and joined the Bioengineering and Physical Science Program of NCRR as a Research Fellow in 1997, developing biophysical methods for the study of protein interactions. In 2014 he was appointed an Earl Stadtman Investigator in NIBIB and Chief, Laboratory of Dynamics of Macromolecular Assembly. In 2021, he was granted tenure at NIH.
Research Interests
The Laoratory of Dynamics of Macromolecular Assembly develops biophysical methods to study protein interactions and the assembly of multi-protein complexes. Hallmarks of multi-protein complexes are multi-valent interactions and cooperativity. In the molecular machinery of cellular processes these constitute ubiquitous mechanisms for the integration and transfer of information. Therefore, our focus is on the development of approaches for multi-component systems where several different macromolecular components interact to allow association and dissociation of different co-existing complexes in different states. We are interested in the characterization of the number of assembly states, and their size, shape, and the interaction energetics. Complementary to crystallographic techniques, such solution interaction studies can provide information on the assembly principles of structurally polymorph multi-protein complexes.
Our group has been particularly active in the development of quantitative hydrodynamic methods using sedimentation velocity analytical ultracentrifugation in conjunction with mathematical modeling of reaction/diffusion/sedimentation processes. We have recently embarked on the development of novel fluorescence detection approaches, which allow unprecedented sensitivity and resolution. We are also pursuing other techniques, including optical biosensing, isothermal titration calorimetry and global multi-method modeling approaches. We are dedicated to the dissemination of methodology, and have implemented our computational analysis methods in widely used software.
We are involved in several collaborative applications in various fields including immunological protein complexes, viral proteins, membrane receptor complexes, and eye lens crystallins.
Publications
The dynamic and heterogeneous structure of the non-canonical inflammasome.
Sever AIMAramini JMBonin JPZhao HWang HRubinstein JLSchuck PKay LE
Proc Natl Acad Sci U S A
2026 Aug 11
The dynamic and heterogeneous structure of the non-canonical inflammasome.
Sever AIMAramini JMBonin JPZhao HWang HRubinstein JLSchuck PKay LE
bioRxiv
2026 Apr 16
Intramolecular loops control SARS-CoV-2 nucleocapsid protein self-association and nucleic acid binding dependent on phosphorylation.
Nguyen ADatta SAKTrent CWu DSaleem ZSzczesna EOwoborode FLi YAdly ANKalish HRPiszczek GMorgan DOSchuck PZhao H
bioRxiv
2026 Jan 12
Evolution of a fuzzy ribonucleoprotein complex in viral assembly.
Zhao HLi THassan SANguyen ADatta SAKZhang GTrent CCzaja AMWu DAronova MALai KKPiszczek GLeapman RYewdell JWSchuck P
Elife
2025 Dec 30
Evolution of a fuzzy ribonucleoprotein complex in viral assembly.
Zhao HLi THassan SANguyen ADatta SAKZhang GTrent CCzaja AMWu DAronova MALai KKPiszczek GLeapman RDYewdell JWSchuck P
bioRxiv
2025 Nov 06
Reply.
Magone MTSchuck P
Cornea
2024 Oct 01
Flotation Coefficient Distributions of Lipid Nanoparticles by Sedimentation Velocity Analytical Ultracentrifugation.
Zhao HSousa AASchuck P
ACS Nano
2024 Jul 16
Modulation of biophysical properties of nucleocapsid protein in the mutant spectrum of SARS-CoV-2.
Nguyen AZhao HMyagmarsuren DSrinivasan SWu DChen JPiszczek GSchuck P
Elife
2024 Jun 28
Assembly of SARS-CoV-2 nucleocapsid protein with nucleic acid.
Zhao HSyed AMKhalid MMNguyen ACiling AWu DYau WMSrinivasan SEsposito DDoudna JAPiszczek GOtt MSchuck P
Nucleic Acids Res
2024 Jun 24
Exploring Host-Guest Interactions within a 600 kDa DegP Protease Cage Complex Using Hydrodynamics Measurements and Methyl-TROSY NMR.
Harkness RWZhao HToyama YSchuck PKay LE
J Am Chem Soc
2024 Mar 27
Amyloid-β aggregates activate peripheral monocytes in mild cognitive impairment.
Juul-Madsen KParbo PIsmail ROvesen PLSchmidt VMadsen LSThyrsted JGierl SBreum MLarsen AAndersen MNRomero-Ramos MHolm CKAndersen GRZhao HSchuck PNygaard JVSutherland DSEskildsen SFWillnow TEBrooks DJVorup-Jensen T
Nat Commun
2024 Feb 09
Modulation of Biophysical Properties of Nucleocapsid Protein in the Mutant Spectrum of SARS-CoV-2.
Nguyen AZhao HMyagmarsuren DSrinivasan SWu DChen JPiszczek GSchuck P
bioRxiv
2024 Mar 22
Evaluating Moisture Migration in Schirmer Test Strips: Exploring Brand-Specific Variations and Introducing Calibration and Conversion Methods.
Magone MTSchuck P
Cornea
2024 May 01
The 5α condensate state in Ne.
Zhou BFunaki YHoriuchi HMa YGRöpke GSchuck PTohsaki AYamada T
Nat Commun
2023 Dec 11
Assembly reactions of SARS-CoV-2 nucleocapsid protein with nucleic acid.
Zhao HSyed AMKhalid MMNguyen ACiling AWu DYau WMSrinivasan SEsposito DDoudna JAPiszczek GOtt MSchuck P
bioRxiv
2023 Nov 23
Diversity of short linear interaction motifs in SARS-CoV-2 nucleocapsid protein.
Schuck PZhao H
mBio
2023 Dec 19
Diversity of Short Linear Interaction Motifs in SARS-CoV-2 Nucleocapsid Protein.
Schuck PZhao H
bioRxiv
2023 Aug 01
An automated interface for sedimentation velocity analysis in SEDFIT.
Schuck PTo SCZhao H
PLoS Comput Biol
2023 Sep
An automated interface for sedimentation velocity analysis in SEDFIT.
Schuck PTo SCZhao H
bioRxiv
2023 May 14
A conserved oligomerization domain in the disordered linker of coronavirus nucleocapsid proteins.
Zhao HWu DHassan SANguyen AChen JPiszczek GSchuck P
Sci Adv
2023 Apr 05
Crk proteins activate the Rap1 guanine nucleotide exchange factor C3G by segregated adaptor-dependent and -independent mechanisms.
Rodríguez-Blázquez ACarabias AMorán-Vaquero Ade Cima SLuque-Ortega JRAlfonso CSchuck PManso JAMacedo-Ribeiro SGuerrero Cde Pereda JM
Cell Commun Signal
2023 Feb 03
Plasticity in structure and assembly of SARS-CoV-2 nucleocapsid protein.
Zhao HNguyen AWu DLi YHassan SAChen JShroff HPiszczek GSchuck P
PNAS Nexus
2022 May
Plasticity in structure and assembly of SARS-CoV-2 nucleocapsid protein.
Zhao HNguyen AWu DLi YHassan SAChen JShroff HPiszczek GSchuck P
bioRxiv
2022 Feb 09
Best Practices for Aggregate Quantitation of Antibody Therapeutics by Sedimentation Velocity Analytical Ultracentrifugation.
Bou-Assaf GMBudyak ILBrenowitz MDay ESHayes DHill JMajumdar RRinghieri PSchuck PLin JC
J Pharm Sci
2022 Jul
The intrinsic kinase activity of BRD4 spans its BD2-B-BID domains.
Weissman JDSingh AKDevaiah BNSchuck PLaRue RCSinger DS
J Biol Chem
2021 Nov
Competing stress-dependent oligomerization pathways regulate self-assembly of the periplasmic protease-chaperone DegP.
Harkness RWToyama YRipstein ZAZhao HSever AIMLuan QBrady JPClark PLSchuck PKay LE
Proc Natl Acad Sci U S A
2021 Aug 10
A multi-step nucleation process determines the kinetics of prion-like domain phase separation.
Martin EWHarmon TSHopkins JBChakravarthy SIncicco JJSchuck PSoranno AMittag T
Nat Commun
2021 Jul 23
Characterization of DNA-protein complexes by nanoparticle tracking analysis and their association with systemic lupus erythematosus.
Juul-Madsen KTroldborg AWittenborn TRAxelsen MGZhao HKlausen LHLuecke SPaludan SRStengaard-Pedersen KDong MMøller HJThiel SJensen HSchuck PSutherland DSDegn SEVorup-Jensen T
Proc Natl Acad Sci U S A
2021 Jul 27
Biomolecular interactions of ultrasmall metallic nanoparticles and nanoclusters.
Sousa AASchuck PHassan SA
Nanoscale Adv
2021 Apr 28
Energetic and structural features of SARS-CoV-2 N-protein co-assemblies with nucleic acids.
Zhao HWu DNguyen ALi YAdão RCValkov EPatterson GHPiszczek GSchuck P
iScience
2021 Jun 25
A multi-laboratory benchmark study of isothermal titration calorimetry (ITC) using Ca and Mg binding to EDTA.
Velazquez-Campoy AClaro BAbian OHöring JBourlon LClaveria-Gimeno REnnifar EEngland PChaires JBWu DPiszczek GBrautigam CTso SCZhao HSchuck PKeller SBastos M
Eur Biophys J
2021 May
Determining the Stoichiometry of a Protein-Polymer Conjugate Using Multisignal Sedimentation Velocity Analytical Ultracentrifugation.
Clardy SMLee DHSchuck P
Bioconjug Chem
2021 May 19
Global multi-method analysis of interaction parameters for reversibly self-associating macromolecules at high concentrations.
Parupudi AChaturvedi SKAdão RHarkness RWDragulin-Otto SKay LEEsfandiary RZhao HSchuck P
Sci Rep
2021 Mar 11
Energetic and structural features of SARS-CoV-2 N-protein co-assemblies with nucleic acids.
Zhao HWu DNguyen ALi YAdão RCValkov EPatterson GHPiszczek GSchuck P
bioRxiv
2021 Feb 09
Calibrating analytical ultracentrifuges.
Zhao HNguyen ATo SCSchuck P
Eur Biophys J
2021 May
An intrinsically disordered motif regulates the interaction between the p47 adaptor and the p97 AAA+ ATPase.
Conicella AEHuang RRipstein ZANguyen AWang ELöhr TSchuck PVendruscolo MRubinstein JLKay LE
Proc Natl Acad Sci U S A
2020 Oct 20
Measuring aggregates, self-association, and weak interactions in concentrated therapeutic antibody solutions.
Chaturvedi SKParupudi AJuul-Madsen KNguyen AVorup-Jensen TDragulin-Otto SZhao HEsfandiary RSchuck P
MAbs
2020
Quantitative Analysis of Protein Self-Association by Sedimentation Velocity.
Zhao HLi WChu WBollard MAdão RSchuck P
Curr Protoc Protein Sci
2020 Sep
Ultrasmall Gold Nanoparticles Coated with Zwitterionic Glutathione Monoethyl Ester: A Model Platform for the Incorporation of Functional Peptides.
Knittel LLZhao HNguyen AMiranda ASchuck PSousa AA
J Phys Chem B
2020 May 14
The biofilm adhesion protein Aap from forms zinc-dependent amyloid fibers.
Yarawsky AEJohns SLSchuck PHerr AB
J Biol Chem
2020 Apr 03
Nucleic acid-induced dimerization of HIV-1 Gag protein.
Zhao HDatta SAKKim SHTo SCChaturvedi SKRein ASchuck P
J Biol Chem
2019 Nov 08
Efficient data acquisition with three-channel centerpieces in sedimentation velocity.
Juul-Madsen KZhao HVorup-Jensen TSchuck P
Anal Biochem
2019 Dec 01
All tubulins are not alike: Heterodimer dissociation differs among different biological sources.
Montecinos-Franjola FChaturvedi SKSchuck PSackett DL
J Biol Chem
2019 Jun 28
Enhanced Sample Handling for Analytical Ultracentrifugation with 3D-Printed Centerpieces.
To SCBrautigam CAChaturvedi SKBollard MTKrynitsky JKakareka JWPohida TJZhao HSchuck P
Anal Chem
2019 May 07
Allosteric inhibition of α-thrombin enzymatic activity with ultrasmall gold nanoparticles.
Lira ALFerreira RSTorquato RJSOliva MLVSchuck PSousa AA
Nanoscale Adv
2019 Jan 01
A formally exact one-frequency-only Bethe-Salpeter-like equation. Similarities and differences between GW+BSE and self-consistent RPA.
Olevano VToulouse JSchuck P
J Chem Phys
2019 Feb 28
A Reappraisal of Sedimentation Nonideality Coefficients for the Analysis of Weak Interactions of Therapeutic Proteins.
Chaturvedi SKSchuck P
AAPS J
2019 Feb 27
Measuring Ultra-Weak Protein Self-Association by Non-ideal Sedimentation Velocity.
Chaturvedi SKSagar VZhao HWistow GSchuck P
J Am Chem Soc
2019 Feb 20
Cooperative assembly of a four-molecule signaling complex formed upon T cell antigen receptor activation.
Manna AZhao HWada JBalagopalan LTagad HDAppella ESchuck PSamelson LE
Proc Natl Acad Sci U S A
2018 Dec 18
Role of humoral immunity against hepatitis B virus core antigen in the pathogenesis of acute liver failure.
Chen ZDiaz GPollicino TZhao HEngle RESchuck PShen CHZamboni FLong ZKabat JDe Battista DBock KWMoore INWollenberg KSoto CGovindarajan SKwong PDKleiner DEPurcell RHFarci P
Proc Natl Acad Sci U S A
2018 Nov 27
Structure of mouse protocadherin 15 of the stereocilia tip link in complex with LHFPL5.
Ge JElferich JGoehring AZhao HSchuck PGouaux E
Elife
2018 Aug 02
A radial calibration window for analytical ultracentrifugation.
LeBrun TSchuck PWei RYoon JSDong XMorgan NYFagan JZhao H
PLoS One
2018
Congratulations to Dr. Fumio Arisaka on his 70th birthday.
Schuck P
Biophys Rev
2018 Apr
Binding kinetics of ultrasmall gold nanoparticles with proteins.
Lira ALFerreira RSTorquato RJSZhao HOliva MLVHassan SASchuck PSousa AA
Nanoscale
2018 Feb 15
Preferential assembly of heteromeric kainate and AMPA receptor amino terminal domains.
Zhao HLomash SChittori SGlasser CMayer MLSchuck P
Elife
2017 Oct 23
Use of fluorescence-detected sedimentation velocity to study high-affinity protein interactions.
Chaturvedi SKMa JZhao HSchuck P
Nat Protoc
2017 Sep
Crystal Structure of Chicken γS-Crystallin Reveals Lattice Contacts with Implications for Function in the Lens and the Evolution of the βγ-Crystallins.
Sagar VChaturvedi SKSchuck PWistow G
Structure
2017 Jul 05
An allosteric site in the T-cell receptor Cβ domain plays a critical signalling role.
Natarajan KMcShan ACJiang JKumirov VKWang RZhao HSchuck PTilahun MEBoyd LFYing JBax AMargulies DHSgourakis NG
Nat Commun
2017 May 16
Sedimentation of Reversibly Interacting Macromolecules with Changes in Fluorescence Quantum Yield.
Chaturvedi SKZhao HSchuck P
Biophys J
2017 Apr 11
Measuring Protein Interactions by Optical Biosensors.
Zhao HBoyd LFSchuck P
Curr Protoc Protein Sci
2017 Apr 03
3D-Printing for Analytical Ultracentrifugation.
Desai AKrynitsky JPohida TJZhao HSchuck P
PLoS One
2016
Monochromatic multicomponent fluorescence sedimentation velocity for the study of high-affinity protein interactions.
Zhao HFu YGlasser CAndrade Alba EJMayer MLPatterson GSchuck P
Elife
2016 Jul 20
Higher-order oligomerization promotes localization of SPOP to liquid nuclear speckles.
Marzahn MRMarada SLee JNourse AKenrick SZhao HBen-Nissan GKolaitis RMPeters JLPounds SErrington WJPrivé GGTaylor JPSharon MSchuck POgden SKMittag T
EMBO J
2016 Jun 15
Sedimentation coefficient distributions of large particles.
Schuck P
Analyst
2016 Jul 21
Integration and global analysis of isothermal titration calorimetry data for studying macromolecular interactions.
Brautigam CAZhao HVargas CKeller SSchuck P
Nat Protoc
2016 May
Biointeractions of ultrasmall glutathione-coated gold nanoparticles: effect of small size variations.
Sousa AAHassan SAKnittel LLBalbo AAronova MABrown PHSchuck PLeapman RD
Nanoscale
2016 Mar 28
Tubulin Dimer Reversible Dissociation: AFFINITY, KINETICS, AND DEMONSTRATION OF A STABLE MONOMER.
Montecinos-Franjola FSchuck PSackett DL
J Biol Chem
2016 Apr 22
Interaction of TAPBPR, a tapasin homolog, with MHC-I molecules promotes peptide editing.
Morozov GIZhao HMage MGBoyd LFJiang JDolan MAVenna RNorcross MAMcMurtrey CPHildebrand WSchuck PNatarajan KMargulies DH
Proc Natl Acad Sci U S A
2016 Feb 23
Variable Field Analytical Ultracentrifugation: II. Gravitational Sweep Sedimentation Velocity.
Ma JZhao HSandmaier JAlexander Liddle JSchuck P
Biophys J
2016 Jan 05
AUC and Small-Angle Scattering for Membrane Proteins.
Le Roy AWang KSchaack BSchuck PBreyton CEbel C
Methods Enzymol
2015
Variable-Field Analytical Ultracentrifugation: I. Time-Optimized Sedimentation Equilibrium.
Ma JMetrick MGhirlando RZhao HSchuck P
Biophys J
2015 Aug 18
A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation.
Zhao HGhirlando RAlfonso CArisaka FAttali IBain DLBakhtina MMBecker DFBedwell GJBekdemir ABesong TMBirck CBrautigam CABrennerman WByron OBzowska AChaires JBChaton CTCölfen HConnaghan KDCrowley KACurth UDaviter TDean WLDíez AIEbel CEckert DMEisele LEEisenstein EEngland PEscalante CFagan JAFairman RFinn RMFischle Wde la Torre JGGor JGustafsson HHall DHarding SECifre JGHerr ABHowell EEIsaac RSJao SCJose DKim SJKokona BKornblatt JAKosek DKrayukhina EKrzizike DKusznir EAKwon HLarson ALaue TMLe Roy ALeech APLilie HLuger KLuque-Ortega JRMa JMay CAMaynard ELModrak-Wojcik AMok YFMücke NNagel-Steger LNarlikar GJNoda MNourse AObsil TPark CKPark JKPawelek PDPerdue EEPerkins SJPerugini MAPeterson CLPeverelli MGPiszczek GPrag GPrevelige PERaynal BDRezabkova LRichter KRingel AERosenberg RRowe AJRufer ACScott DJSeravalli JGSolovyova ASSong RStaunton DStoddard CStott KStrauss HMStreicher WWSumida JPSwygert SGSzczepanowski RHTessmer IToth RTTripathy AUchiyama SUebel SFUnzai SGruber AVvon Hippel PHWandrey CWang SHWeitzel SEWielgus-Kutrowska BWolberger CWolff MWright EWu YSWubben JMSchuck P
PLoS One
2015
A histogram approach to the quality of fit in sedimentation velocity analyses.
Ma JZhao HSchuck P
Anal Biochem
2015 Aug 15
Biocalorimetry.
Chaires JBHansen LDKeller SBrautigam CAZhao HSchuck P
Methods
2015 Apr
Combining biophysical methods for the analysis of protein complex stoichiometry and affinity in SEDPHAT.
Zhao HSchuck P
Acta Crystallogr D Biol Crystallogr
2015 Jan 01
SEDPHAT--a platform for global ITC analysis and global multi-method analysis of molecular interactions.
Zhao HPiszczek GSchuck P
Methods
2015 Apr
Accounting for photophysical processes and specific signal intensity changes in fluorescence-detected sedimentation velocity.
Zhao HMa JIngaramo MAndrade EMacDonald JRamsay GPiszczek GPatterson GHSchuck P
Anal Chem
2014 Sep 16
Measurement of the temperature of the resting rotor in analytical ultracentrifugation.
Ghirlando RZhao HBalbo APiszczek GCurth UBrautigam CASchuck P
Anal Biochem
2014 Aug 01
A structural framework for a near-minimal form of life: mass and compositional analysis of the helical mollicute Spiroplasma melliferum BC3.
Trachtenberg SSchuck PPhillips TMAndrews SBLeapman RD
PLoS One
2014
Analysis of protein interactions with picomolar binding affinity by fluorescence-detected sedimentation velocity.
Zhao HMayer MLSchuck P
Anal Chem
2014 Mar 18
Improved measurement of the rotor temperature in analytical ultracentrifugation.
Zhao HBalbo AMetger HClary RGhirlando RSchuck P
Anal Biochem
2014 Apr 15
Human herpesvirus 7 U21 tetramerizes to associate with class I major histocompatibility complex molecules.
May NAWang QBalbo AKonrad SLBuchli RHildebrand WHSchuck PHudson AW
J Virol
2014 Mar
Solution properties of γ-crystallins: hydration of fish and mammal γ-crystallins.
Zhao HChen YRezabkova LWu ZWistow GSchuck P
Protein Sci
2014 Jan
SEDFIT-MSTAR: molecular weight and molecular weight distribution analysis of polymers by sedimentation equilibrium in the ultracentrifuge.
Schuck PGillis RBBesong TMAlmutairi FAdams GGRowe AJHarding SE
Analyst
2014 Jan 07
Solution properties of γ-crystallins: compact structure and low frictional ratio are conserved properties of diverse γ-crystallins.
Chen YZhao HSchuck PWistow G
Protein Sci
2014 Jan
Analysis of high affinity self-association by fluorescence optical sedimentation velocity analytical ultracentrifugation of labeled proteins: opportunities and limitations.
Zhao HLomash SGlasser CMayer MLSchuck P
PLoS One
2013
Complexes of neutralizing and non-neutralizing affinity matured Fabs with a mimetic of the internal trimeric coiled-coil of HIV-1 gp41.
Gustchina ELi MGhirlando RSchuck PLouis JMPierson JRao PSubramaniam SGustchina AClore GMWlodawer A
PLoS One
2013
Tools for the quantitative analysis of sedimentation boundaries detected by fluorescence optical analytical ultracentrifugation.
Zhao HCasillas EShroff HPatterson GHSchuck P
PLoS One
2013
Multipoint binding of the SLP-76 SH2 domain to ADAP is critical for oligomerization of SLP-76 signaling complexes in stimulated T cells.
Coussens NPHayashi RBrown PHBalagopalan LBalbo AAkpan IHoutman JCBarr VASchuck PAppella ESamelson LE
Mol Cell Biol
2013 Nov
Analysis of high-affinity assembly for AMPA receptor amino-terminal domains.
Zhao HBerger AJBrown PHKumar JBalbo AMay CACasillas ELaue TMPatterson GHMayer MLSchuck P
J Gen Physiol
2013 Jun
Improving the thermal, radial, and temporal accuracy of the analytical ultracentrifuge through external references.
Ghirlando RBalbo APiszczek GBrown PHLewis MSBrautigam CASchuck PZhao H
Anal Biochem
2013 Sep 01
Multi-signal sedimentation velocity analysis with mass conservation for determining the stoichiometry of protein complexes.
Brautigam CAPadrick SBSchuck P
PLoS One
2013
Analytical Ultracentrifugation as a Tool for Studying Protein Interactions.
Schuck P
Biophys Rev
2013 Jun 01
Biophysical methods for the study of protein interactions.
Schuck PZhao H
Methods
2013 Mar
A conformational switch in HP1 releases auto-inhibition to drive heterochromatin assembly.
Canzio DLiao MNaber NPate ELarson AWu SMarina DBGarcia JFMadhani HDCooke RSchuck PCheng YNarlikar GJ
Nature
2013 Apr 18
Recorded scan times can limit the accuracy of sedimentation coefficients in analytical ultracentrifugation.
Zhao HGhirlando RPiszczek GCurth UBrautigam CASchuck P
Anal Biochem
2013 Jun 01
Overview of current methods in sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation.
Zhao HBrautigam CAGhirlando RSchuck P
Curr Protoc Protein Sci
2013 Feb
A comparison of binding surfaces for SPR biosensing using an antibody-antigen system and affinity distribution analysis.
Zhao HGorshkova IIFu GLSchuck P
Methods
2013 Mar
Global multi-method analysis of affinities and cooperativity in complex systems of macromolecular interactions.
Zhao HSchuck P
Anal Chem
2012 Nov 06
Strategies for assessing proton linkage to bimolecular interactions by global analysis of isothermal titration calorimetry data.
Coussens NPSchuck PZhao H
J Chem Thermodyn
2012 Sep 01
High-precision isothermal titration calorimetry with automated peak-shape analysis.
Keller SVargas CZhao HPiszczek GBrautigam CASchuck P
Anal Chem
2012 Jun 05
Analysis of high-affinity assembly for AMPA receptor amino-terminal domains.
Zhao HBerger AJBrown PHKumar JBalbo AMay CACasillas ELaue TMPatterson GHMayer MLSchuck P
J Gen Physiol
2012 May
Structural and thermodynamic characterization of the interaction between two periplasmic Treponema pallidum lipoproteins that are components of a TPR-protein-associated TRAP transporter (TPAT).
Brautigam CADeka RKSchuck PTomchick DRNorgard MV
J Mol Biol
2012 Jun 29
An equilibrium model for linear and closed-loop amyloid fibril formation.
Yang SGriffin MDBinger KJSchuck PHowlett GJ
J Mol Biol
2012 Aug 10
Structural, bioinformatic, and in vivo analyses of two Treponema pallidum lipoproteins reveal a unique TRAP transporter.
Deka RKBrautigam CAGoldberg MSchuck PTomchick DRNorgard MV
J Mol Biol
2012 Mar 09
Density contrast sedimentation velocity for the determination of protein partial-specific volumes.
Brown PHBalbo AZhao HEbel CSchuck P
PLoS One
2011
NBD-labeled phospholipid accelerates apolipoprotein C-II amyloid fibril formation but is not incorporated into mature fibrils.
Ryan TMGriffin MDBailey MFSchuck PHowlett GJ
Biochemistry
2011 Nov 08
Malaria vaccine candidate: design of a multivalent subunit α-helical coiled coil poly-epitope.
Olugbile SVillard VBertholet SJafarshad AKulangara CRoussilhon CFrank GAgak GWFelger INebie IKonate KKajava AVSchuck PDruilhe PSpertini FCorradin G
Vaccine
2011 Sep 16
Structure and assembly mechanism for heteromeric kainate receptors.
Kumar JSchuck PMayer ML
Neuron
2011 Jul 28
The molecular refractive function of lens γ-Crystallins.
Zhao HBrown PHMagone MTSchuck P
J Mol Biol
2011 Aug 19
The role of macromolecular crowding in the evolution of lens crystallins with high molecular refractive index.
Zhao HMagone MTSchuck P
Phys Biol
2011 Aug
On the distribution of protein refractive index increments.
Zhao HBrown PHSchuck P
Biophys J
2011 May 04
Editorial for the special issue of methods "Modern Analytical Ultracentrifugation".
Schuck PZhao H
Methods
2011 May
Dimeric states of neural- and epithelial-cadherins are distinguished by the rate of disassembly.
Vunnam NFlint JBalbo ASchuck PPedigo S
Biochemistry
2011 Apr 12
The boundary structure in the analysis of reversibly interacting systems by sedimentation velocity.
Zhao HBalbo ABrown PHSchuck P
Methods
2011 May
Extended Fujita approach to the molecular weight distribution of polysaccharides and other polymeric systems.
Harding SESchuck PAbdelhameed ASAdams GKök MSMorris GA
Methods
2011 May
Biophysical characterization of DNA and RNA aptamer interactions with hen egg lysozyme.
Potty ASKourentzi KFang HSchuck PWillson RC
Int J Biol Macromol
2011 Apr 01
Cooperative interactions at the SLP-76 complex are critical for actin polymerization.
Barda-Saad MShirasu NPauker MHHassan NPerl OBalbo AYamaguchi HHoutman JCAppella ESchuck PSamelson LE
EMBO J
2010 Jul 21
Diffusion of the reaction boundary of rapidly interacting macromolecules in sedimentation velocity.
Schuck P
Biophys J
2010 Jun 02
Accounting for solvent signal offsets in the analysis of interferometric sedimentation velocity data.
Zhao HBrown PHBalbo AFernández-Alonso Mdel CPolishchuck NChaudhry CMayer MLGhirlando RSchuck P
Macromol Biosci
2010 Jul 07
Sedimentation patterns of rapidly reversible protein interactions.
Schuck P
Biophys J
2010 May 19
Phospholipids enhance nucleation but not elongation of apolipoprotein C-II amyloid fibrils.
Ryan TMTeoh CLGriffin MDBailey MFSchuck PHowlett GJ
J Mol Biol
2010 Jun 25
The role of mass transport limitation and surface heterogeneity in the biophysical characterization of macromolecular binding processes by SPR biosensing.
Schuck PZhao H
Methods Mol Biol
2010
Some statistical properties of differencing schemes for baseline correction of sedimentation velocity data.
Schuck P
Anal Biochem
2010 Jun 15
Direct interaction of the mouse cytomegalovirus m152/gp40 immunoevasin with RAE-1 isoforms.
Zhi LMans JPaskow MJBrown PHSchuck PJonjić SNatarajan KMargulies DH
Biochemistry
2010 Mar 23
On computational approaches for size-and-shape distributions from sedimentation velocity analytical ultracentrifugation.
Schuck P
Eur Biophys J
2010 Jul
Assembly, loading, and alignment of an analytical ultracentrifuge sample cell.
Balbo AZhao HBrown PHSchuck P
J Vis Exp
2009 Nov 05
On the analysis of sedimentation velocity in the study of protein complexes.
Brown PHBalbo ASchuck P
Eur Biophys J
2009 Oct
Energetics of glutamate receptor ligand binding domain dimer assembly are modulated by allosteric ions.
Chaudhry CPlested AJSchuck PMayer ML
Proc Natl Acad Sci U S A
2009 Jul 28
Novel chimpanzee/human monoclonal antibodies that neutralize anthrax lethal factor, and evidence for possible synergy with anti-protective antigen antibody.
Chen ZMoayeri MCrown DEmerson SGorshkova ISchuck PLeppla SHPurcell RH
Infect Immun
2009 Sep
The N-terminal domain of GluR6-subtype glutamate receptor ion channels.
Kumar JSchuck PJin RMayer ML
Nat Struct Mol Biol
2009 Jun
The C-terminal domain of Plasmodium falciparum merozoite surface protein 3 self-assembles into alpha-helical coiled coil tetramer.
Gondeau CCorradin GHeitz FLe Peuch CBalbo ASchuck PKajava AV
Mol Biochem Parasitol
2009 Jun
Stability of ligand-binding domain dimer assembly controls kainate receptor desensitization.
Chaudhry CWeston MCSchuck PRosenmund CMayer ML
EMBO J
2009 May 20
Autoinhibition of Arf GTPase-activating protein activity by the BAR domain in ASAP1.
Jian XBrown PSchuck PGruschus JMBalbo AHinshaw JERandazzo PA
J Biol Chem
2009 Jan 16
Bayesian analysis of heterogeneity in the distribution of binding properties of immobilized surface sites.
Gorshkova IISvitel JRazjouyan FSchuck P
Langmuir
2008 Oct 21
A bayesian approach for quantifying trace amounts of antibody aggregates by sedimentation velocity analytical ultracentrifugation.
Brown PHBalbo ASchuck P
AAPS J
2008 Sep
Characterizing protein-protein interactions by sedimentation velocity analytical ultracentrifugation.
Brown PHBalbo ASchuck P
Curr Protoc Immunol
2008 May
Humanized monoclonal antibodies derived from chimpanzee Fabs protect against Japanese encephalitis virus in vitro and in vivo.
Goncalvez APChien CHTubthong KGorshkova IRoll CDonau OSchuck PYoksan SWang SDPurcell RHLai CJ
J Virol
2008 Jul
Apolipoprotein C-II amyloid fibrils assemble via a reversible pathway that includes fibril breaking and rejoining.
Binger KJPham CLWilson LMBailey MFLawrence LJSchuck PHowlett GJ
J Mol Biol
2008 Feb 29
A new adaptive grid-size algorithm for the simulation of sedimentation velocity profiles in analytical ultracentrifugation.
Brown PHSchuck P
Comput Phys Commun
2008 Jan 15
Bacteriorhodopsin/amphipol complexes: structural and functional properties.
Gohon YDahmane TRuigrok RWSchuck PCharvolin DRappaport FTimmins PEngelman DMTribet CPopot JLEbel C
Biophys J
2008 May 01
Extended polypeptide linkers establish the spatial architecture of a pyruvate dehydrogenase multienzyme complex.
Lengyel JSStott KMWu XBrooks BRBalbo ASchuck PPerham RNSubramaniam SMilne JL
Structure
2008 Jan
Measuring protein-protein interactions by equilibrium sedimentation.
Balbo ABrown PHBraswell EHSchuck P
Curr Protoc Immunol
2007 Nov
Two interferons alpha influence each other during their interaction with the extracellular domain of human type interferon receptor subunit 2.
Schmeisser HGorshkova IBrown PHKontsek PSchuck PZoon KC
Biochemistry
2007 Dec 18
Cellular expression and crystal structure of the murine cytomegalovirus major histocompatibility complex class I-like glycoprotein, m153.
Mans JNatarajan KBalbo ASchuck PEikel DHess SRobinson HSimic HJonjic STiemessen CTMargulies DH
J Biol Chem
2007 Nov 30
Characterization of chimpanzee/human monoclonal antibodies to vaccinia virus A33 glycoprotein and its variola virus homolog in vitro and in a vaccinia virus mouse protection model.
Chen ZEarl PAmerico JDamon ISmith SKYu FSebrell AEmerson SCohen GEisenberg RJGorshkova ISchuck PSatterfield WMoss BPurcell R
J Virol
2007 Sep
Using prior knowledge in the determination of macromolecular size-distributions by analytical ultracentrifugation.
Brown PHBalbo ASchuck P
Biomacromolecules
2007 Jun
Single-walled tubulin ring polymers.
Boukari HSackett DLSchuck PNossal RJ
Biopolymers
2007
Superantigen natural affinity maturation revealed by the crystal structure of staphylococcal enterotoxin G and its binding to T-cell receptor Vbeta8.2.
Fernández MMBhattacharya SDe Marzi MCBrown PHKerzic MSchuck PMariuzza RAMalchiodi EL
Proteins
2007 Jul 01
Studying multisite binary and ternary protein interactions by global analysis of isothermal titration calorimetry data in SEDPHAT: application to adaptor protein complexes in cell signaling.
Houtman JCBrown PHBowden BYamaguchi HAppella ESamelson LESchuck P
Protein Sci
2007 Jan
Probing the functional heterogeneity of surface binding sites by analysis of experimental binding traces and the effect of mass transport limitation.
Svitel JBoukari HVan Ryk DWillson RCSchuck P
Biophys J
2007 Mar 01
Measuring protein interactions by optical biosensors.
Schuck PBoyd LFAndersen PS
Curr Protoc Cell Biol
2004 May
Case histories illustrating the utility of sertindole in clinical practice.
Schuck Pvan den Ameele HJaanson PRyckmans VHawley C
CNS Drugs
2004
SDR grafting of a murine antibody using multiple human germline templates to minimize its immunogenicity.
Gonzales NRPadlan EADe Pascalis RSchuck PSchlom JKashmiri SV
Mol Immunol
2004 Jul
Crystal structure of the C-terminal peptidoglycan-binding domain of human peptidoglycan recognition protein Ialpha.
Guan RMalchiodi ELWang QSchuck PMariuzza RA
J Biol Chem
2004 Jul 23
Assessing reproducibility for interval data in health-related quality of life questionnaires: which coefficient should be used?
Schuck P
Qual Life Res
2004 Apr
Calculating sedimentation coefficient distributions by direct modeling of sedimentation velocity concentration profiles.
Dam JSchuck P
Methods Enzymol
2004
Binding specificity of multiprotein signaling complexes is determined by both cooperative interactions and affinity preferences.
Houtman JCHigashimoto YDimasi NCho SYamaguchi HBowden BRegan CMalchiodi ELMariuzza RSchuck PAppella ESamelson LE
Biochemistry
2004 Apr 13
A model for sedimentation in inhomogeneous media. II. Compressibility of aqueous and organic solvents.
Schuck P
Biophys Chem
2004 Mar 01
A model for sedimentation in inhomogeneous media. I. Dynamic density gradients from sedimenting co-solutes.
Schuck P
Biophys Chem
2004 Mar 01
Sedimentation equilibrium analysis of protein interactions with global implicit mass conservation constraints and systematic noise decomposition.
Vistica JDam JBalbo AYikilmaz EMariuzza RARouault TASchuck P
Anal Biochem
2004 Mar 15
Conformational restriction blocks glutamate receptor desensitization.
Weston MCSchuck PGhosal ARosenmund CMayer ML
Nat Struct Mol Biol
2006 Dec
Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1.
Houtman JCYamaguchi HBarda-Saad MBraiman ABowden BAppella ESchuck PSamelson LE
Nat Struct Mol Biol
2006 Sep
Variable dimerization of the Ly49A natural killer cell receptor results in differential engagement of its MHC class I ligand.
Dam JBaber JGrishaev AMalchiodi ELSchuck PBax AMariuzza RA
J Mol Biol
2006 Sep 08
Macromolecular size-and-shape distributions by sedimentation velocity analytical ultracentrifugation.
Brown PHSchuck P
Biophys J
2006 Jun 15
Quaternary structure and cleavage specificity of a poxvirus holliday junction resolvase.
Garcia ADOtero JLebowitz JSchuck PMoss B
J Biol Chem
2006 Apr 28
Crystal structure of group A streptococcus Mac-1: insight into dimer-mediated specificity for recognition of human IgG.
Agniswamy JNagiec MJLiu MSchuck PMusser JMSun PD
Structure
2006 Feb
Efficient neutralization of anthrax toxin by chimpanzee monoclonal antibodies against protective antigen.
Chen ZMoayeri MZhou YHLeppla SEmerson SSebrell AYu FSvitel JSchuck PSt Claire MPurcell R
J Infect Dis
2006 Mar 01
Chimpanzee/human mAbs to vaccinia virus B5 protein neutralize vaccinia and smallpox viruses and protect mice against vaccinia virus.
Chen ZEarl PAmerico JDamon ISmith SKZhou YHYu FSebrell AEmerson SCohen GEisenberg RJSvitel JSchuck PSatterfield WMoss BPurcell R
Proc Natl Acad Sci U S A
2006 Feb 07
Influence of temperature on the conformation of canine plasminogen: an analytical ultracentrifugation and dynamic light scattering study.
Kornblatt JASchuck P
Biochemistry
2005 Oct 04
Dissection of merozoite surface protein 3, a representative of a family of Plasmodium falciparum surface proteins, reveals an oligomeric and highly elongated molecule.
Burgess BRSchuck PGarboczi DN
J Biol Chem
2005 Nov 04
Regulation of ASAP1 by phospholipids is dependent on the interface between the PH and Arf GAP domains.
Che MMBoja ESYoon HYGruschus JJaffe HStauffer SSchuck PFales HMRandazzo PA
Cell Signal
2005 Oct
Self-association and ligand-induced conformational changes of iron regulatory proteins 1 and 2.
Yikilmaz ERouault TASchuck P
Biochemistry
2005 Jun 14
A mechanism for assembly of complexes of vitronectin and plasminogen activator inhibitor-1 from sedimentation velocity analysis.
Minor KHSchar CRBlouse GEShore JDLawrence DASchuck PPeterson CB
J Biol Chem
2005 Aug 05
Sedimentation velocity analysis of heterogeneous protein-protein interactions: Lamm equation modeling and sedimentation coefficient distributions c(s).
Dam JVelikovsky CAMariuzza RAUrbanke CSchuck P
Biophys J
2005 Jul
Sedimentation velocity analysis of heterogeneous protein-protein interactions: sedimentation coefficient distributions c(s) and asymptotic boundary profiles from Gilbert-Jenkins theory.
Dam JSchuck P
Biophys J
2005 Jul
Eukaryotic RNases H1 act processively by interactions through the duplex RNA-binding domain.
Gaidamakov SAGorshkova IISchuck PSteinbach PJYamada HCrouch RJCerritelli SM
Nucleic Acids Res
2005
Characterization of DC-SIGN/R interaction with human immunodeficiency virus type 1 gp120 and ICAM molecules favors the receptor's role as an antigen-capturing rather than an adhesion receptor.
Snyder GAFord JTorabi-Parizi PArthos JASchuck PColonna MSun PD
J Virol
2005 Apr
Cooperative behavior of Escherichia coli cell-division protein FtsZ assembly involves the preferential cyclization of long single-stranded fibrils.
González JMVélez MJiménez MAlfonso CSchuck PMingorance JVicente MMinton APRivas G
Proc Natl Acad Sci U S A
2005 Feb 08
Targeted lysis of HIV-infected cells by natural killer cells armed and triggered by a recombinant immunoglobulin fusion protein: implications for immunotherapy.
Gupta NArthos JKhazanie PSteenbeke TDCensoplano NMChung EACruz CCChaikin MADaucher MKottilil SMavilio DSchuck PSun PDRabin RLRadaev SVan Ryk DCicala CFauci AS
Virology
2005 Feb 20
Exposure of acidic residues as a danger signal for recognition of fibrinogen and other macromolecules by integrin alphaXbeta2.
Vorup-Jensen TCarman CVShimaoka MSchuck PSvitel JSpringer TA
Proc Natl Acad Sci U S A
2005 Feb 01
Studying multiprotein complexes by multisignal sedimentation velocity analytical ultracentrifugation.
Balbo AMinor KHVelikovsky CAMariuzza RAPeterson CBSchuck P
Proc Natl Acad Sci U S A
2005 Jan 04
In vitro affinity maturation of a specificity-determining region-grafted humanized anticarcinoma antibody: isolation and characterization of minimally immunogenic high-affinity variants.
De Pascalis RGonzales NRPadlan EASchuck PBatra SKSchlom JKashmiri SV
Clin Cancer Res
2003 Nov 15
Variable MHC class I engagement by Ly49 natural killer cell receptors demonstrated by the crystal structure of Ly49C bound to H-2K(b).
Dam JGuan RNatarajan KDimasi NChlewicki LKKranz DMSchuck PMargulies DHMariuzza RA
Nat Immunol
2003 Dec
Minimizing immunogenicity of the SDR-grafted humanized antibody CC49 by genetic manipulation of the framework residues.
Gonzales NRPadlan EADe Pascalis RSchuck PSchlom JKashmiri SV
Mol Immunol
2003 Oct
Reversible and fast association equilibria of a molecular chaperone, gp57A, of bacteriophage T4.
Ali SAIwabuchi NMatsui THirota KKidokoro SArai MKuwajima KSchuck PArisaka F
Biophys J
2003 Oct
On the analysis of protein self-association by sedimentation velocity analytical ultracentrifugation.
Schuck P
Anal Biochem
2003 Sep 01
The 'smallest real difference' as a measure of sensitivity to change: a critical analysis.
Schuck PZwingmann C
Int J Rehabil Res
2003 Jun
Alanine-scanning mutations in domain 4 of anthrax toxin protective antigen reveal residues important for binding to the cellular receptor and to a neutralizing monoclonal antibody.
Rosovitz MJSchuck PVarughese MChopra APMehra VSingh YMcGinnis LMLeppla SH
J Biol Chem
2003 Aug 15
Combined affinity and rate constant distributions of ligand populations from experimental surface binding kinetics and equilibria.
Svitel JBalbo AMariuzza RAGonzales NRSchuck P
Biophys J
2003 Jun
Inhibition of hemostasis by a high affinity biogenic amine-binding protein from the saliva of a blood-feeding insect.
Andersen JFFrancischetti IMValenzuela JGSchuck PRibeiro JM
J Biol Chem
2003 Feb 14
Differences in the binding capacity of human apolipoprotein E3 and E4 to size-fractionated lipid emulsions.
Perugini MASchuck PHowlett GJ
Eur J Biochem
2002 Dec
Grafting of "abbreviated" complementarity-determining regions containing specificity-determining residues essential for ligand contact to engineer a less immunogenic humanized monoclonal antibody.
De Pascalis RIwahashi MTamura MPadlan EAGonzales NRSantos ADGiuliano MSchuck PSchlom JKashmiri SV
J Immunol
2002 Sep 15
Surface plasmon resonance-based competition assay to assess the sera reactivity of variants of humanized antibodies.
Gonzales NRSchuck PSchlom JKashmiri SV
J Immunol Methods
2002 Oct 15
Modern analytical ultracentrifugation in protein science: a tutorial review.
Lebowitz JLewis MSSchuck P
Protein Sci
2002 Sep
Epidemiology research.
Schuck P
Lancet
2002 Jul 20
Analysis of a temperature-sensitive mutant rotavirus indicates that NSP2 octamers are the functional form of the protein.
Taraporewala ZFSchuck PRamig RFSilvestri LPatton JT
J Virol
2002 Jul
Mass spectrometry after capture and small-volume elution of analyte from a surface plasmon resonance biosensor.
Gilligan JJSchuck PYergey AL
Anal Chem
2002 May 01
Quantifying the energetics of cooperativity in a ternary protein complex.
Andersen PSSchuck PSundberg EJGeisler CKarjalainen KMariuzza RA
Biochemistry
2002 Apr 23
Size-distribution analysis of proteins by analytical ultracentrifugation: strategies and application to model systems.
Schuck PPerugini MAGonzales NRHowlett GJSchubert D
Biophys J
2002 Feb
Biochemical and biological characterization of a dodecameric CD4-Ig fusion protein: implications for therapeutic and vaccine strategies.
Arthos JCicala CSteenbeke TDChun TWDela Cruz CHanback DBKhazanie PNam DSchuck PSelig SMVan Ryk DChaikin MAFauci AS
J Biol Chem
2002 Mar 29
Elucidating kinetic and thermodynamic constants for interaction of G protein subunits and receptors by surface plasmon resonance spectroscopy.
Rebois RVSchuck PNorthup JK
Methods Enzymol
2002