Immunohistochemistry

Pathology Support Group

The Immunohistochemistry Support Team performs immunohistochemical stains on tissues and cells for NIEHS Division of Intramural Research and Division of Translational Toxicology researchers.

Immunohistochemistry Support Staff


Renee D. Fisher
Renee D. Fisher Supervisory Biologist Tel 984-287-3956 renee.fisher@nih.gov

Heather L. Jensen
Heather L. Jensen, M.S. Lead Biologist Tel 984-287-3930 jensen@niehs.nih.gov

Mark A. Rubino
Mark A. Rubino Biologist Tel 984-287-3934 rubinom@niehs.nih.gov

Charan K. Ganta
Charan K. Ganta, B.V.Sc., Ph.D., D.A.C.V.P. Contractor - IHC Pathologist Tel 984-287-3833 charan.ganta@nih.gov

David J. Olson
David J. Olson Contractor Tel 984-287-3933 olsond2@niehs.nih.gov

Tara W. Moulden
Tara W. Moulden Contractor Tel 984-287-3932 tara.moulden@nih.gov

Capabilities

Highly trained staff members provide the following key functions:

  • Develop staining protocols for tissue preserved in a variety of fixatives.
    • 10% neutral buffered formalin, 4% paraformaldehyde, ethanol, methanol.
  • Enzyme based visualization using brown, red, blue, and purple chromogenic substrates.
  • Offer antibody selection assistance.
  • Perform co-localization experiments using chromogen or fluorescent detection.
  • Perform cryopreservation and sectioning of frozen tissue samples.
  • Perform immunohistochemical stains on formalin-fixed paraffin-embedded tissue or OCT embedded frozen tissue sections and cytology samples.
  • Perform Immunofluorescence on paraffin-embedded or frozen tissue sections.
  • Perform single or dual localization.
  • Provide information on tissue handling and preparation that promote optimal staining results.
  • Provide pathology reports.
  • Provide routine antibodies for IHC staining.
  • Work closely with NIEHS researchers to develop and optimize staining procedures for novel and commercially available antibodies.

Routine Antibodies provided by the Immunohistochemistry (IHC) Laboratory

  • beta catenin
  • BrdU
  • caspase-3
  • CD3
  • ED2
  • F4/80
  • Factor VIII
  • Iba1
  • Ki67
  • p53
  • pancytokeratin
  • Pax-5
  • PCNA
  • TUNEL
  • vimentin

Immunohistochemistry in Investigative and Toxicologic Pathology Immunohistochemistry Protocols Reagents Retrievals